|
KMID : 0390320110210020139
|
|
Chungbuk Medical Journal
2011 Volume.21 No. 2 p.139 ~ p.147
|
|
|
Dendritic distribution of mRNA candidates
|
|
Kwon A-Ra
Jo An-Na
Kim Hyong-Kyu
|
|
|
Abstract
|
|
|
Purpose: The purpose of this research was to examine the dendritic localization of mRNA candidates, which had been isolated from Staufen-immunoprecipitates.
Materials and Methods : We performed in situ hybridization or fluorescent in situ hybridization assays accompanied with MAP2 immunostaining, probing Agrin, eIF6, Lsm12, Piwi-like1, or Sec14- like3 mRNA, in cultured hippocampal neurons.
Results: All the in situ signals of Agrin, eIF6, Lsm12, Piwi-like1, and Sec14-like3 mRNAs were found in dendrites. The signals of eIF6 and Sec14-like3 were detected with more higher rate than others in dendrites.
Conclusion: These results suggest a possibility that Agrin, eIF6, Lsm12, Piwi-like1, and Sec14- like3 proteins might be regulated by the local protein synthesis.
|
|
|
KEYWORD
|
|
|
Local protein synthesis, In situ hybridization, Image analysis, Staufen
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|